A psychiatrist tells two stories: one of a trans woman, the other of a pseudohermaphrodite.
Bela Lugosi
Scientist
Edward D. Wood Jr.
Glen / Glenda (as Daniel Davis)
Lyle Talbot
Inspector Warren
Timothy Farrell
Dr. Alton / Narrator
Dolores Fuller
Barbara
'Tommy' Haynes
Alan / Anne
Charlie Crafts
Johnny (as Charles Crafts)
Conrad Brooks
Banker / Reporter / Pickup Artist / Bearded Drag (as Connie Brooks)
Henry Bederski
Man with Hat and Receding Hairline (uncredited)
Carol Daugherty
Woman in Nightmare (uncredited)
'Captain' DeZita
The Devil / Glen's Father (uncredited)
Helen Miles
(uncredited)
Shirley Speril
Miss Stevens (uncredited)
Amzie Strickland
Minor Role (uncredited)
Harry Thomas
Man in Nightmare (uncredited)
William C. Thompson
Judge (uncredited)
'Mr. Walter' Hadjwiecyz
Patrick / Patricia (uncredited)
George Weiss
Man at Transvestite's Suicide (uncredited)
Evelyn Wood
Sheila, Glen's Sister (uncredited)